Identify the steps taken in the Show more Preparation of a bacteria smear and the simple stain technique 1) Identify the steps taken in the preparation of a bacterial smear to prevent contamination of the culture and yourself working with a bacterial smear? 2) Summarize the fundamental theory of simple staining. hint: what scientific property allows the dyes t bind the bacterial cell wall? 3) What would happen if you placed a large drop of water on a slide rather than a loopful when preparing a bacterial smear? 4) Discuss the possible outcomes if an air-dried smear were not heat fixed before proceeding to the staining step. 5) While viewing a slide of a pure culture you observe two different shaped bacteria; some rod shaped while others are spherical. How could this happen? List at least two explanations and/or sources for this result. Show less
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