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How will you know that vector taken up by bacteria contains an insertion of foreign DNA?
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June 13, 2022Diagram the 4 essential components of a bacterial Cloning Vector (plasmid). How would you insert you Show more Diagram the 4 essential components of a bacterial Cloning Vector (plasmid). How would you insert your DNA/gene of interest (such as a human or other mammalian gene) into this bacterial DNA carrier? How will you know that recipient bacteria have taken-up your vector? How will you know that vector taken up by bacteria contains an insertion of foreign DNA? How would you identify which recombinant vector has your particular gene/sequence of interest? Show less
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